Physico-chemical and biotic factors influencing microalgal seed culture propagation for inoculation of a large scale raceway pond
Ranjith Kumar, R.
MetadataShow full item record
The growth of Chlorella vulgaris in open pond aquatic conditions poses serious challenges due to the interplay of both physico-chemical and biotic factors. We report here the monitoring of physico-chemical and biotic parameters affecting the propagation of C. vulgaris seed culture for inoculation of a large scale raceway pond (300 000 L capacity) in South Africa. The C. vulgarisstrain used for this purpose was isolated from a wastewater maturation pond and characterized for its potential for biomass and lipid production. The isolate was grown aseptically in 4 × 25 L aspirator bottles in BG-11 medium under ambient laboratory conditions and the culture was supplied with filtered air and exposed to 200 µmol photons per m2 per second using Gro-Lux agricultural fluorescent lights. The culture was transferred to a 500 L capacity portable pool under open conditions. This pond was used to further inoculate 3 more portable ponds. Physico-chemical and biotic growth parameters were monitored on a daily basis in the three ponds. The over reliance on fossil fuels will have a major impact on power supply in the near future if renewable sources of energy are not developed at a fast pace. The developed inoculum was subsequently used to inoculate an open raceway pond for large scale biomass production for biodiesel production.
Mutanda, T, Ranjith, R.K. and Bux, F. 2014. Physico-chemical and biotic factors influencing microalgal seed culture propagation for inoculation of a large scale raceway pond. African Journal of Biotechnology. 13(31): 3607-3616.