Exploring the potential reservoirs of non speciﬁc TEM beta lactamase (blaTEM) gene in the Indo-Gangetic region : a risk assessment approach to predict health hazards
Amoah, Isaac Dennis
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Copyright: 2016. Elsevier. Due to copyright restrictions, only the abstract is available. For access to the full text item, please consult the publisher's website. The definitive version of the work is published in Journal of Hazardous Materials, Vol 314. Pp 121-128. https://www.ncbi.nlm.nih.gov/pubmed/27111425
The emergence of antimicrobial resistant bacteria is an important public health and environ-mental contamination issue. Antimicrobials of -lactam group accounts for approximately two thirds, by weight, of all antimicrobials administered to humans due to high clinical efﬁcacy and low toxicity. This study explores -lactam resistance determinant gene (blaTEM) as emerging con-taminant in Indo-Gangetic region using qPCR in molecular beacon format. Quantitative Microbial Risk Assessment (QMRA) approach was adopted to predict risk to human health associated with consumption/exposure of surface water, potable water and street foods contaminated with bac-teria having blaTEM gene. It was observed that surface water and sediments of the river Ganga and Gomti showed high numbers of blaTEM gene copies and varied signiﬁcantly (p < 0.05) among the sampling locations. The potable water collected from drinking water facility and clinical set-tings exhibit signiﬁcant number of blaTEM gene copies (13 ± 0.44–10200 ± 316 gene copies/100 mL).
Singh, G. et al. 2016. Exploring the potential reservoirs of non speciﬁc TEM beta lactamase (blaTEM) gene in the Indo-Gangetic region : a risk assessment approach to predict health hazards. Journal of Hazardous Materials. 314: 121-128.