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dc.contributor.authorPienaar, Fernando
dc.date.accessioned2008-07-16T12:46:25Z
dc.date.available2008-07-16T12:46:25Z
dc.date.issued2007
dc.identifier.other308421
dc.identifier.urihttp://hdl.handle.net/10321/307
dc.descriptionThesis (M.Tech.: Biotechnology)-Dept. of Biotechnology and Food Technology, Durban University of Technology, 2007 iv, 75 leavesen_US
dc.description.abstractThe development of transgenic plants resulted in the need to utilize the various molecular methods (e.g., ELISA, real - time PCR etc.) for the detection or analysis of the presence or absence of a specific trait in a particular plant (Bt in this study). The overall aim of this study was to optimize a half – seed extraction technique as part of a laboratory protocol for transgenic maize plants and to explore the possibility of using the following molecular techniques: horizontal isoelectric focusing, real - time PCR and ELISA, as methods for detection of the Bt trait for incorporation into the half – seed extraction protocol.en_US
dc.language.isoenen_US
dc.subjectCorn--Genetic engineeringen_US
dc.subjectCrops--Genetic engineeringen_US
dc.subjectCorn--Biotechnologyen_US
dc.subjectPlant genetic engineeringen_US
dc.subjectCrop improvementen_US
dc.subjectBiotechnology--Dissertations, Academicen_US
dc.titleComparative analysis of genetically modified maize by implementation of a half-seed extraction techniqueen_US
dc.typeThesisen_US


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