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Comparative analysis of genetically modified maize by implementation of a half-seed extraction technique

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dc.contributor.author Pienaar, Fernando
dc.date.accessioned 2008-07-16T12:46:25Z
dc.date.available 2008-07-16T12:46:25Z
dc.date.issued 2007
dc.identifier.other 308421
dc.identifier.uri http://hdl.handle.net/10321/307
dc.description Thesis (M.Tech.: Biotechnology)-Dept. of Biotechnology and Food Technology, Durban University of Technology, 2007 iv, 75 leaves en_US
dc.description.abstract The development of transgenic plants resulted in the need to utilize the various molecular methods (e.g., ELISA, real - time PCR etc.) for the detection or analysis of the presence or absence of a specific trait in a particular plant (Bt in this study). The overall aim of this study was to optimize a half – seed extraction technique as part of a laboratory protocol for transgenic maize plants and to explore the possibility of using the following molecular techniques: horizontal isoelectric focusing, real - time PCR and ELISA, as methods for detection of the Bt trait for incorporation into the half – seed extraction protocol. en_US
dc.language.iso en en_US
dc.subject Corn--Genetic engineering en_US
dc.subject Crops--Genetic engineering en_US
dc.subject Corn--Biotechnology en_US
dc.subject Plant genetic engineering en_US
dc.subject Crop improvement en_US
dc.subject Biotechnology--Dissertations, Academic en_US
dc.title Comparative analysis of genetically modified maize by implementation of a half-seed extraction technique en_US
dc.type Thesis en_US


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