Show simple item record

dc.contributor.authorNaidoo, Dashika
dc.date.accessioned2008-07-18T08:06:21Z
dc.date.available2008-07-18T08:06:21Z
dc.date.issued2005
dc.identifier.otherDIT112628
dc.identifier.urihttp://hdl.handle.net/10321/313
dc.descriptionThesis (M.Tech.: Biotechnology)-Dept. of Biotechnology, Durban Institute of Technology, 2005 xiv, 114 leaves : ill. 30 cmen_US
dc.description.abstractThe activated sludge process is the mostl used biological treatment process. Engineers and microbiologists are constantly seeking ways to improve process efficiency, which can be attributed to the increasing demand for fresh water supplies and proper environmental management. Since the inception of the activated sludge process, bulking and foaming have been major problems affecting its efficiency. Filamentous bacteria have been identified as the primary cause of bulking and foaming. Numerous attempts have been made to resolve this problem. Some of these attempts were effective as interim measures but failed as long term control strategies. The identification of filamentous bacteria and the study of their physiology have been hampered by the unreliability of conventional microbiological techniques. This is largely due to their morphological variations and inconsistent characteristics within different environments. To fully understand their role in promoting bulking and foaming, filamentous bacteria need to be characterized on a molecular level. The aim of this study was, therefore, to identify filamentous bacteria in pure culture with the purpose of validating these findings to the physiological traits of the pure cultures when they were isolated. Fourteen different filamentous cultures were used for this study. The cultures were identified using specific oligonucleotide probes via fluorescent in situ hybridisation and nucleotide sequencing. Prior to sequencing, an agarose gel and a denaturing gradient gel Electrophoresis profile were determined for each isolate. The various techniques were optimised specifically for the filamentous isolates. The isolates were identified as Gordonia amarae, Haliscomenobacter hydrossis, Acinetobacter sp./Type 1863, Type 021N, Thiothrix nivea, Sphaerotilus natans and Nocardioform organisms.en_US
dc.language.isoenen_US
dc.subjectBiotechnology--Dissertations, Academicen_US
dc.subjectSewage--Purification--Activated sludge processen_US
dc.subjectSanitary microbiology--Dissertations, Academicen_US
dc.subjectSewage--Purification--Biological treatmenten_US
dc.titleMolecular analyses of pure cultures of filamentous bacteria isolated from activated sludgeen_US
dc.typeThesisen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record