A comparative study of Hahnemannian and radionically prepared potencies of Natrum muriaticum using nuclear magnetic resonance spectroscopy
The aim of this study was to compare the Nuclear Magnetic Resonance (NMR) spectra of homoeopathic potencies prepared according to the orthodox Hahnemannian method with those produced with Radionic instrumentation. Methodology The chemical shift values and relative integration values for the H2O, CH2, CH3 and OH peaks of the 6C, 12C and 30C potencies of Hahnemannian and Radionic Natrum muriaticum were compared. The orthodox Hahnemannian method of preparing potencies involves dilution of the crude substance followed by the dilution and succussion at each subsequent deconcentration (potency) level. The Hahnemannian potencies were prepared according to the German Homoeopathic Pharmacopoeia (GHP) and the potencies diluted using a 1:100 ratio and succussed ten times at each potency level. The Radionic group of potencies were prepared using the ‘Magnetogeometric Potency Simulator’ (a Radionic apparatus). NMR testing took place at the Chemistry Department at the University of KwaZulu Natal, Pietermaritzburg using a Bruker Avance lll NMR spectrometer 500MHz.The samples were dispensed into boro-silicate glass NMR tubes with a co-axial tube containing Dimethyl sulfoxide-d6 (DMSO-d6) which was used as a frequency lock around the tube. Three samples were drawn from each group, including the controls, and analysed using the NMR spectrometer. The NMR spectrometer information was received and the chemical shift and relative integration values of H2O, OH, CH2 and CH3 peaks on the NMR spectra recorded. All the data was entered into a Microsoft Excel© 2000 spreadsheet and then from there transferred into SPSS© software package for statistical analysis. The Kruskal-Wallis test was used to make a comparison between the eight unpaired groups. If a significant difference occurred between the groups iii individual comparisons between groups were made using the non-parametric Mann-Whitney test. The significant value was set at α= 0.05. Results The results of this study revealed significant differences between the Hahnemannian and Radionic samples. The chemical shift values of the parallel potencies showed significant differences for the H2O, CH2 and CH3 peaks. A significant difference for the OH peaks was observed between the 30C potencies. The relative integration values showed a significant difference for the OH and CH3 peaks between the parallel 12C and 30C potencies but not between the parallel 6C potencies. Conclusion From studying the results it can be concluded that the respective manufacture methods resulted in the NMR spectra of the parallel potencies being significantly different (exhibiting distinctive physico-chemical properties) thus confirming the hypotheses of the study. The standardisation of the process of preparing homoeopathic remedies is important as different methods produce potencies with distinct physico-chemical identities. Further studies into different methods should be researched in order to control and standardise the production of potencies.