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Enhanced production of inulinase from Xanthomonas campestris pv. phaseoli

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dc.contributor.advisor Singh, Suren
dc.contributor.advisor Permaul, Kugen Naidoo, Kameshnee 2011-03-31T07:44:22Z 2012-09-01T22:20:07Z 2010
dc.identifier.other 332261
dc.description Submitted in complete fulfillment for the Degree of Master of Technology: Biotechnology, Durban University of Technology, 2010. en_US
dc.description.abstract Xanthomonas campestris pv phaseoli produced an extracellular endoinulinase on various carbon sources. The highest inulinase production of 9.24 ± 0.03 IU ml¯¹by X. campestris pv. phaseoli was attained using an optimized medium comprising of 3% sucrose and 2.5% tryptone. Inulinase production in X. campestris pv. phaseoli was further enhanced through ethylmethanesulfonate mutagenesis. The resulting mutant, X. campestris pv. phaseoli KM 24 demonstrated enhanced inulinase production of 22.09 ± 0.03 IU ml¯¹after 24 h, which was 2.4 – fold higher than that of the wild type. Inulinase production by this mutant was scaled up in a 5 L fermenter yielding a final activity of 21.87 ± 0.03 IU ml¯¹with an inulinase/invertase (I/S) ratio of 2.6 after 18 h. Maximum volumetric (21 865 IU 1¯¹ h¯¹) and specific (119 025 IU g¯¹ h¯¹) productivities of inulinase were attained in a fermenter after 18h growth. Inulin hydrolysis by the crude inulinase and subsequent detection of mono- and oligosaccharides indicated the presence of an endoinulinase. The extracellular endoinulinase from the mutant KM 24 was purified to homogeneity by gel filtration chromatography and had a specific activity of 174.74U/mg. the optimum pH and temperature of the purified enzyme were found to be 6.0 and 50°C, respectively. The enzyme was stable up to 60°C, retaining over 60% activity for 30 min, but activity rapidly declined at temperatures above 60°C. The pure inulinase enzyme was also found to be stable between pH 6-9. The Lineweaver-Burk plots showed that the apparent Km and Vmax values of the inulinase for inulin were 1.15 mg/ml and 15µM/min, respectively. The Kcat value was found to be 0.145 min¯¹ with an enzyme catalytic efficiency of 0.126 mg¯¹.ml.min¯¹.This mutant demonstrated good potential for large scale production of inulinase and fructooligosaccharides. en_US
dc.description.sponsorship National Research Foundation en_US
dc.format.extent 101 p en_US
dc.language.iso en en_US
dc.subject.lcsh Inulin en_US
dc.subject.lcsh Xanthomonas campestris en_US
dc.subject.lcsh Hydrolysis en_US
dc.subject.lcsh Industrial microbiology en_US
dc.subject.lcsh Enzymes--Industrial applications en_US
dc.subject.lcsh Biotechnology en_US
dc.title Enhanced production of inulinase from Xanthomonas campestris pv. phaseoli en_US
dc.type Thesis en_US
dc.dut-rims.pubnum DUT-002230

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