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Immunomodulatory activities of non-commercialized leafy vegetables in KwaZulu-Natal, South Africa

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dc.contributor.advisor Odhav, Bharti
dc.contributor.advisor Reddy, Lalini
dc.contributor.author Padayachee, Berushka
dc.date.accessioned 2012-05-25T11:15:28Z
dc.date.available 2014-02-11T12:32:58Z
dc.date.issued 2012
dc.identifier.other 418262
dc.identifier.uri http://hdl.handle.net/10321/722
dc.description Submitted in complete fulfillment of the requirements for the Degree of Master of Technology: Biotechnology, Durban University of Technology, 2012. en_US
dc.description.abstract Immunomodulation using plants is of primary interest in scientific communities because it provides an alternative to conventional chemotherapy for a wide range of diseases. It is based on the ability of the plants to effectively modulate immune functions, thus being able to promote positive health and maintain the body’s resistance to infection. This research is aimed to evaluate the immunomodulatory potential of fourteen traditional leafy vegetables from Kwa-Zulu Natal, South Africa on human peripheral blood mononuclear cells (PBMC). In this study the methanolic and aqueous extracts were screened for lymphocyte proliferation using the MTT assay. The cytokine response was evaluated by measuring the secretion of interleukin 10 (IL-10) and interferon-gamma (IFN-γ) using the ELISA assay. The subpopulation of T cells viz., CD4+, CD8+, NK and B cells were measured by flow cytometry. Most of the methanolic extracts stimulated PBMC’s whilst a few suppressed lymphocyte proliferation. Most of the aqueous extracts were inactive. The methanolic extracts of Amaranthus hybridus and Centella asiatica stimulated PBMC’s and showed an increase in IFN-γ secretion and the CD8+ cytotoxic T cells and B cells. Thus, they induced the Tc-1 immune response and stimulated cell mediated immunity. The methanolic extracts of Asystasia gangetica, Bidens pilosa, Emex australis, Justicia flava Momordica balsamina, Oxygonum sinuatum, Senna occidentalis and Sonchus oleraceous and the aqueous extracts of Amaranthus spinosus and Asystasia gangetica, Ceratotheca triloba, Oxygonum sinuatum, Physalis viscosa and Sonchus oleaceous stimulated PBMC’s and showed an increase in IL-10 secretion and the CD8+ cytotoxic T cells and B cells. Thus, they induced the Tc-2 immune response and stimulated humoral immunity. Also, the methanolic extracts of Amaranthus spinosus and Ceratotheca triloba and the aqueous extracts of Bidens pilosa and Justicia flava increased both IL-10 and IFN-γ secretion and the CD8+ vii cytotoxic T cells indicating the stimulation of both the Tc1 and Tc2 cytokine profiles. The elevated secretion of IFN-γ and IL-10 caused by the extracts can be attributed to the CD8+ cytotoxic T cells and B cells. The findings of this study show that leafy vegetables hold promise as immunomodulatory candidates. They may enhance cell-mediated immune functions by a pro-inflammatory response whilst some can promote humoral immune functions by means of an anti-inflammatory response. Further investigation should be considered on the effect of the extracts on other immune parameters. en_US
dc.description.sponsorship National Research Foundation en_US
dc.format.extent 170 p en_US
dc.language.iso en en_US
dc.subject.lcsh Biotechnology en_US
dc.subject.lcsh Immune response--Regulation en_US
dc.subject.lcsh Edible greens--South Africa--KwaZulu-Natal en_US
dc.title Immunomodulatory activities of non-commercialized leafy vegetables in KwaZulu-Natal, South Africa en_US
dc.type Thesis en_US
dc.dut-rims.pubnum DUT-002246
dc.dut-rims.pubnum DUT-002246


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