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Microbial community analysis of a laboratory-scale biological process for the treatment of vegetable oil effluent

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dc.contributor.advisor Bux, Faizal
dc.contributor.author Degenaar, Adrian Phillip
dc.date.accessioned 2012-06-29T08:19:38Z
dc.date.available 2014-02-11T12:32:57Z
dc.date.issued 2011
dc.identifier.other 418883
dc.identifier.uri http://hdl.handle.net/10321/733
dc.description Dissertation submitted in fulfilment with the requirements for the Masters Degree: Biotechnology, Durban University of Technology, 2011. en_US
dc.description.abstract Untreated vegetable oil effluents (VOEs) are known for creating shock-loading problems for the receiving wastewater treatment installations, resulting in poor quality final effluents being produced which do not satisfy municipal discharge standards. Onsite activated sludge treatment as an alternative has not been fully investigated. Hence, in this investigation biological treatment using the activated sludge process was chosen as the method for the treatment of VOE. The effect of VOE on measured process parameters was also determined. Novel molecular techniques such as fluorescent in situ hybridisation (FISH) and dot-blot hybridization have become powerful tools for the analysis of complex microbial communities that exist within activated sludge. The aim of this investigation was to evaluate biological treatment, optimize and apply FISH and dot-blot hybridization in order to analyze the microbial community implicated the biological treatment of VOE using probes EUBmix, ALF1b, BET42a, GAM42a and HGC69a. A laboratory-scale modified Ludzack-Ettinger (MLE) process setup and fed VOE with a COD (chemical oxygen demand) of ± 1000 mg/L. Daily monitoring of the process involved COD and TKN (total kjeldahl nitrogen) analysis of the influent and effluent as well as direct OUR (oxygen utilization rate) measurement and monitoring of the MLVSS (mixed liquor volatile suspended solids) concentration of the aerobic mixed liquor. The process exhibited overall COD and TKN removal capacities of 84% and 90% respectively. The aerobic mixed liquor had an OUR of 19 mgO/L.h and an average MLVSS concentration of 3000 mg/L. FISH results revealed that 72% of cells stained with 4‟, 6-diamidino-2-phenylindole (DAPI) within the aerobic mixed liquor bound to probe EUBmix, indicating a substantial Bacterial population within the laboratory-scale biological process. The alpha-Proteobacteria was identified as the dominant bacterial community comprising 31% of Bacterial cells, followed by the beta-Proteobacteria (17% of EUBmix), gamma-Proteobacteria (8% of EUBmix) and Actinobacteria (4% of EUBmix). Results of dot-blot hybridization were in agreement with FISH Adrian Phillip Degenaar| CHAPTER 1: General Introduction - v - results reiterating dominance of the alpha-Proteobacteria. This indicated that the class alpha-Proteobacteria could play a primary role in the biological degradation of VOE. This research will therefore aid in process design and retrofitting of biological processes treating VOE. en_US
dc.format.extent 167 p en_US
dc.language.iso en en_US
dc.subject Vegetable oil effluent en_US
dc.subject Ludzack-Ettinger en_US
dc.subject Chemical oxygen demand en_US
dc.subject Total Kjeldahl nitrogen en_US
dc.subject.lcsh Sewage--Purification--Biological treatment en_US
dc.subject.lcsh Vegetable oil industry--Waste disposal en_US
dc.subject.lcsh Sewage--Purification--Activated sludge process en_US
dc.subject.lcsh Fluorescence in situ hybridization en_US
dc.title Microbial community analysis of a laboratory-scale biological process for the treatment of vegetable oil effluent en_US
dc.type Thesis en_US
dc.dut-rims.pubnum DUT-000203


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