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|Title:||Exploring the potential reservoirs of non speciﬁc TEM beta lactamase (blaTEM) gene in the Indo-Gangetic region : a risk assessment approach to predict health hazards||Authors:||Singh, Gulshan
Amoah, Isaac Dennis
|Keywords:||Environmental reservoirs;blaTEM gene;Molecular beacon;Quantitative PCR;β-lactamase resistance||Issue Date:||2016||Publisher:||Elsevier||Source:||Singh, G. et al. 2016. Exploring the potential reservoirs of non speciﬁc TEM beta lactamase (blaTEM) gene in the Indo-Gangetic region : a risk assessment approach to predict health hazards. Journal of Hazardous Materials. 314: 121-128.||Journal:||Journal of hazardous materials (Online) ItemCrisRefDisplayStrategy.journals.deleted.icon||Abstract:||The emergence of antimicrobial resistant bacteria is an important public health and environ-mental contamination issue. Antimicrobials of -lactam group accounts for approximately two thirds, by weight, of all antimicrobials administered to humans due to high clinical efﬁcacy and low toxicity. This study explores -lactam resistance determinant gene (blaTEM) as emerging con-taminant in Indo-Gangetic region using qPCR in molecular beacon format. Quantitative Microbial Risk Assessment (QMRA) approach was adopted to predict risk to human health associated with consumption/exposure of surface water, potable water and street foods contaminated with bac-teria having blaTEM gene. It was observed that surface water and sediments of the river Ganga and Gomti showed high numbers of blaTEM gene copies and varied signiﬁcantly (p < 0.05) among the sampling locations. The potable water collected from drinking water facility and clinical set-tings exhibit signiﬁcant number of blaTEM gene copies (13 ± 0.44–10200 ± 316 gene copies/100 mL).||URI:||http://hdl.handle.net/10321/2461||ISSN:||0304-3894 (print)
|Appears in Collections:||Research Publications (Academic Support)|
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