Please use this identifier to cite or link to this item:
https://hdl.handle.net/10321/1483
DC Field | Value | Language |
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dc.contributor.author | Ayyappa, Bathinapatla | |
dc.contributor.author | Kanchi, Suvardhan | |
dc.contributor.author | Singh, Parvesh | |
dc.contributor.author | Dovey, Martin | |
dc.contributor.author | Sabela, Myalowenkosi Innocent | |
dc.contributor.author | Bisetty, Krishna | |
dc.date.accessioned | 2016-05-05T08:51:48Z | |
dc.date.available | 2016-05-05T08:51:48Z | |
dc.date.issued | 2014-05-05 | |
dc.identifier.citation | Ayyappa, B.; Kanchi, S.; Singh, P.; Sabela, M. I.; Dovey, M. and Bisetty, K. 2014. Analytical evaluation of steviol glycosides by capillary electrophoresis supported with molecular docking studies. Journal of the Iranian Chemical Society, 12 (1): 127-136. | en_US |
dc.identifier.issn | 1735-2428 | |
dc.identifier.uri | http://hdl.handle.net/10321/1483 | |
dc.description.abstract | This paper reports on a newly developed elec-trokinetic chromatographic method for the simultaneous separation and determination of steviol glycosides in real stevia samples by capillary electrophoresis and supported by molecular docking studies. Our results obtained using 30-mM heptakis-(2,3,6-tri-o-methyl betacyclodextrin) as a separating agent, suggest that at optimum experimental conditions the detection limits of 2.017 9 10-5 and 7.386 9 10-5 M and relative standard deviations (n = 5) of 1.10 and 1.17 were obtained for rebaudioside-A and stevioside, respectively. In addition, the molecular docking studies explained to a certain extent why the separation was successful. The calculated binding free energy results for the rebaudioside-A and stevioside complexes formed with the separating agent showed that although both ligands penetrated deeply into the hydrophobic cavity of the sep-arating agent, the presence of additional hydrogen bonding in the case of stevioside is probably responsible for its stronger binding affinity than that of rebaudioside-A. | en_US |
dc.format.extent | 10 p | en_US |
dc.language.iso | en | en_US |
dc.publisher | Iranian Chemical Society | en_US |
dc.relation.ispartof | Journal of the Iranian Chemical Society (Online) | - |
dc.subject | Steviol glycosides (rebaudioside-A, stevioside) | en_US |
dc.subject | Heptakis 2,3,6-tri-o-methyl betacyclodextrin (TM-b-CD) | en_US |
dc.subject | Capillary electrophoresis (CE) | en_US |
dc.subject | Molecular docking (MD) | en_US |
dc.title | Analytical evaluation of steviol glycosides by capillary electrophoresis supported with molecular docking studies | en_US |
dc.type | Article | en_US |
dc.publisher.uri | http://link.springer.com/article/10.1007/s13738-014-0465-z/fulltext.html | en_US |
dc.dut-rims.pubnum | DUT-004046 | en_US |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | Article | - |
Appears in Collections: | Research Publications (Applied Sciences) |
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File | Description | Size | Format | |
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Ayyappa_JICS_12_1_014.pdf | 1.02 MB | Adobe PDF | View/Open |
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