Please use this identifier to cite or link to this item: https://hdl.handle.net/10321/1653
DC FieldValueLanguage
dc.contributor.authorXiao, Yanen_US
dc.contributor.authorShen, Weien_US
dc.contributor.authorGovender, Algasanen_US
dc.contributor.authorZhang, Liangen_US
dc.contributor.authorXianzhong, Chenen_US
dc.date.accessioned2016-10-13T06:30:43Z-
dc.date.available2016-10-13T06:30:43Z-
dc.date.issued2016-
dc.identifier.citationXianzhong, C. et a. 2016. Display of phytase on the cell surface of Saccharomyces cerevisiae to degrade phytate phosphorus and rove bioethanol production. Bioenergy and Biofuels. 100: 2449–2458en_US
dc.identifier.issn0175-7598-
dc.identifier.urihttp://hdl.handle.net/10321/1653-
dc.description.abstractCurrently, development of biofuels as an alternative fuel has gained much attention due to resource and environ-mental challenges. Bioethanol is one of most important and dominant biofuels, and production using corn or cassava as raw materials has become a prominent technology. However, phytate contained in the raw material not only decreases the efficiency of ethanol production, but also leads to an increase in the discharge of phosphorus, thus impacting on the environment. In this study, to decrease phytate and its phos-phorus content in an ethanol fermentation process, Saccharomyces cerevisiae was engineered through a surface-displaying system utilizing the C-terminal half of the yeast α-agglutinin protein. The recombinant yeast strain, PHY, was constructed by successfully displaying phytase on the surface of cells, and enzyme activity reached 6.4 U/g wet biomass weight. Ethanol productions using various strains were com-pared, and the results demonstrated that the specific growth rate and average fermentation rate of the PHY strain were higher 20 and 18 %, respectively, compared to the control strain S. cerevisiae CICIMY0086, in a 5-L bioreactor process by simultaneous saccharification and fermentation. More im-portantly, the phytate phosphorus concentration decreased by 89.8 % and free phosphorus concentration increased by 142.9 % in dry vinasse compared to the control in a 5-L bio-reactor. In summary, we constructed a recombinant S. cerevisiae strain displaying phytase on the cell surface, which could improve ethanol production performance and effectively reduce the discharge of phosphorus. The strain reported here represents a useful novel engineering platform for developing an environment-friendly system for bioethanol production from a corn substrate.en_US
dc.format.extent10 pen_US
dc.language.isoenen_US
dc.publisherSpringer Verlagen_US
dc.relation.ispartofApplied microbiology and biotechnologyen_US
dc.subjectBioethanolen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectPhytaseen_US
dc.subjectSurface-displaying expressionen_US
dc.titleDisplay of phytase on the cell surface of Saccharomyces cerevisiae to degrade phytate phosphorus and improve bioethanol productionen_US
dc.typeArticleen_US
dc.publisher.urihttp://download.springer.com/static/pdf/748/art%253A10.1007%252Fs00253-015-7170-4.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00253-015-7170-4&token2=exp=1476268162~acl=%2Fstatic%2Fpdf%2F748%2Fart%25253A10.1007%25252Fs00253-015-7170-4.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Farticle%252F10.1007%252Fs00253-015-7170-4*~hmac=ff802cd34b36ba1f77d87d25a504ca7d34cd332477949bee0468e0e9980088aden_US
dc.dut-rims.pubnumDUT-005195en_US
dc.description.availabilityCopyright: 2016. Springer Verlag. Due to copyright restrictions, only the abstract is available. For access to the full text item, please consult the publisher's website. The definitive version of the work is published in Bioenergy and Biofuels, Vol 100, pp 2449-2458.en_US
dc.identifier.doi10.1007/s00253-015-7170-4-
local.sdgSDG07-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
Appears in Collections:Research Publications (Applied Sciences)
Show simple item record

Page view(s)

636
checked on Dec 13, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.