Please use this identifier to cite or link to this item:
https://hdl.handle.net/10321/1745
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Singh, Suren | - |
dc.contributor.advisor | Permaul, Kugen | - |
dc.contributor.advisor | Puri, Adarsh Kumar | - |
dc.contributor.author | Chanderman, Ashira | en_US |
dc.date.accessioned | 2016-11-18T06:50:33Z | - |
dc.date.available | 2016-11-18T06:50:33Z | - |
dc.date.issued | 2016 | - |
dc.identifier.other | 663055 | - |
dc.identifier.uri | http://hdl.handle.net/10321/1745 | - |
dc.description | Submitted in complete fulfillment for the Degree of Master of Applied Sciences in Biotechnology, Durban University of Technology, Durban, South Africa, 2016. | en_US |
dc.description.abstract | A bacterial strain producing an extracellular phytase was identified as Enterobacter sp. ACSS. Optimization of process parameters using statistical methods such as Plackett-Burman design (PBD), the steepest ascent method, and response surface methodology (RSM) significantly improved phytase production by 4.6–fold in shake-flasks. In addition, an overall 1.9-fold increase in phytase production was attained in fed-batch fermentations in a 5 l laboratory fermenter, respectively. The purified 62 kDa phytase from Enterobacter sp. ACSS was active between 40 to 80°C and an acidic pH range of 2.0 to 6.0 with half-life of 693 and 577.5 min at 60°C and pH 2.0, respectively. Additionally, the enzyme is fairly stable with proteolytic enzymes under physiological conditions. It was activated by Ca+2, Mg+2 and Mn+2 while inhibition was caused by Zn+2, Cu+2, Fe+2, Pb+2, Co+2, Ba+2 and surfactants. The Km, Vmax and Kcat observed were 0.21 mM, 131.58 nmol mg-1s-1 and 1.64 × 103 s-1, respectively. The enzyme released inorganic phosphate from animal feed (4.0-6.62 mg/g of diet) and insoluble metal-phytates (45-219 µg/ml) and was effective in improving the characteristics of brown bread. Overall, this study shows that Enterobacter sp. ACSS has the potential to produce significant titres of a thermo- and acid-stable phytase and can be applied in dephytinizing animal feeds, and the baking industry. | en_US |
dc.format.extent | 144 p | en_US |
dc.language.iso | en | en_US |
dc.subject.lcsh | Phytases | en_US |
dc.subject.lcsh | Feeds | en_US |
dc.subject.lcsh | Enterobacter | en_US |
dc.subject.lcsh | Phosphates | en_US |
dc.title | Production, characterisation and applications of a thermo-acid-stable phytase from Enterobacter sp. ACSS | en_US |
dc.type | Thesis | en_US |
dc.description.level | M | en_US |
dc.identifier.doi | https://doi.org/10.51415/10321/1745 | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.openairetype | Thesis | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
Appears in Collections: | Theses and dissertations (Applied Sciences) |
Files in This Item:
File | Description | Size | Format | |
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CHANDERMAN_2016.pdf | 2.95 MB | Adobe PDF | View/Open |
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