Please use this identifier to cite or link to this item: https://hdl.handle.net/10321/2442
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dc.contributor.authorZhou, Junboen_US
dc.contributor.authorChen, Xianzhongen_US
dc.contributor.authorLu, Lipingen_US
dc.contributor.authorGovender, Algasanen_US
dc.contributor.authorHaiquan, Yangen_US
dc.contributor.authorShen, Wieen_US
dc.date.accessioned2017-05-10T06:12:33Z-
dc.date.available2017-05-10T06:12:33Z-
dc.date.issued2016-
dc.identifier.citationZhou, J. et al. 2016. Enhanced production of N-acetyl-d-neuraminic acid by whole-cell bio-catalysis of Escherichia coli. Journal of Molecular Catalysis B: Enzymatic. 125: 42–48.en_US
dc.identifier.issn1381-1177-
dc.identifier.urihttp://hdl.handle.net/10321/2442-
dc.description.abstractN-acetyl-d-neuraminic acid (Neu5Ac) has been considerably focused due to its promising potential appli-cations in pharmaceuticals and dairy products. A whole-cell biocatalyst process is an important tool for synthesis of pharmaceutical intermediates and fine chemicals. In this study, a whole cell process using engineered Escherichia coli strain was developed and stepwise optimization was employed for Neu5Ac production. N-acetyl-D-glucosamine 2-epimerase and Neu5Ac aldolase were overexpressed in E. coli individually and the activity ratio was optimized by varying recombinant amounts of cell biomass for syn-thesis of Neu5Ac. Moreover, substrate concentrations and ratio of pyruvate and N-acetyl-D-glucosamine (GlcNAc) and detergent concentrations were optimized to increase product synthesis. The resulting process generated 237.4 mM Neu5Ac with a yield of 40.0% mol/mol GlcNAc. Furthermore, transporter pathways involved in Neu5Ac and GlcNAc were engineered and their impact on the Neu5Ac synthesis was evaluated. Using a stepwise optimization, an overall whole-cell biocatalytic process was developed and a maximum titer of 260.0 mM Neu5Ac (80.4 g/L) with a conversion yield of 43.3% from GlcNAc was achieved. The process can be used for industrial large-scale production of Neu5Ac in terms of efficiency and economy.en_US
dc.format.extent7 pen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of molecular catalysis. B, Enzymatic (Print)en_US
dc.subjectN-Acetyl-D-neuraminic aciden_US
dc.subjectEscherichia colien_US
dc.subjectWhole-cellbio-catalysisen_US
dc.subjectN-Acetyl-d-glucosamineen_US
dc.subjectStepwise optimizationen_US
dc.titleEnhanced production of N-acetyl-d-neuraminic acid by whole-cell bio-catalysis of Escherichia colien_US
dc.typeArticleen_US
dc.publisher.urihttp://www.sciencedirect.com/science/article/pii/S1381117715301144en_US
dc.dut-rims.pubnumDUT-005655en_US
dc.description.availabilityCopyright: 2015. Elsevier. Due to copyright restrictions, only the abstract is available. For access to the full text item, please consult the publisher's website. The definitive version of the work is published in Journal of Molecular Catalysis B: Enzymatic, Vol 125. Pp 42-48. http://www.sciencedirect.com/science/article/pii/S1381117715301144en_US
dc.identifier.doihttps://doi.org/10.1016/j.molcatb.2015.11.027-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
Appears in Collections:Research Publications (Applied Sciences)
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