Please use this identifier to cite or link to this item: https://hdl.handle.net/10321/2582
DC FieldValueLanguage
dc.contributor.authorSingh, Alveera-
dc.contributor.authorVenugopala, Katharigatta Narayanaswamy-
dc.contributor.authorKhedr, Mohammed A.-
dc.contributor.authorPillay, Mellendran-
dc.contributor.authorNwaeze, Kenneth U.-
dc.contributor.authorCoovadia, Yacoob-
dc.contributor.authorShode, Francis-
dc.contributor.authorOdhav, Bharti-
dc.date.accessioned2017-09-22T08:43:12Z-
dc.date.available2017-09-22T08:43:12Z-
dc.date.issued2017-
dc.identifier.citationSingh, A. 2017. Antimycobacterial, docking and molecular dynamic studies of pentacyclic triterpenes from Buddleja saligna leaves. Journal of Biomolecular Structure & Dynamic. 35(12): 2654-2664.en_US
dc.identifier.issn0739-1102 (print)-
dc.identifier.issn1538-0254 (online)-
dc.identifier.urihttp://hdl.handle.net/10321/2582-
dc.description.abstractBuddleja saligna (family Buddlejaceae) is a medicinal plant endemic to South Africa. Two isomeric pentacyclic triterpenes, oleanolic acid and ursolic acid, were isolated from the leaves of B. saligna using silica gel column chromatography. Compounds oleanolic acid and ursolic acid were subjected to derivatization with acetic anhydride in the presence of pyridine to obtain oleanolic acid-3-acetate and ursolic acid-3-acetate, respectively. The structures of these compounds were fully characterized by detailed nuclear magnetic resonance (NMR) investigations, which included 1H and 13C NMR. Molecular docking studies predicted the free binding energy of the four triterpenes inside the steroid binding pocket of Mycobacterium tuberculosis fadA5 thiolase compared to a reported inhibitor. Thus, their ability to inhibit the growth of M. tuberculosis was predicted and was confirmed to possess significant antimycobacterial activity when tested against Mycobacterium smegmatis, M. tuberculosis H37Rv (ATCC 25177), clinical isolates of multi-drug-resistant M. tuberculosis (MDR-TB) and extensively drug-resistant M. tuberculosis (XDR-TB) using the Micro Alamar Blue Assay. Ursolic acid was isolated from this plant for the first time.en_US
dc.format.extent11 pen_US
dc.language.isoenen_US
dc.publisherTaylor and Fancis Onlineen_US
dc.relation.ispartofJournal of biomolecular structure and dynamics (Online)-
dc.subjectAntimycobacterial activityen_US
dc.subjectBuddleja salignaen_US
dc.subjectDockingen_US
dc.subjectfadA5 thiolaseen_US
dc.subjectOleanolic aciden_US
dc.subjectPentacyclic triterpenesen_US
dc.subjectUrsolic aciden_US
dc.titleAntimycobacterial, docking and molecular dynamic studies of pentacyclic triterpenes from Buddleja saligna leavesen_US
dc.typeThesisen_US
dc.publisher.urihttp://www.tandfonline.com/doi/abs/10.1080/07391102.2016.1227725?journalCode=tbsd20en_US
dc.dut-rims.pubnumDUT-005761en_US
dc.description.availabilityCopyright: 2016. Taylor & Francis Online. Due to copyright restrictions, only the abstract is available. For access to the full text item, please consult the publisher's website. The definitive version of the work is published in Journal of Biomolecular Structure & Dynamic. Vol 35(12) : 2654-2664 http://www.tandfonline.com/doi/abs/10.1080/07391102.2016.1227725?journalCode=tbsd20en_US
local.sdgSDG03-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
item.openairetypeThesis-
Appears in Collections:Research Publications (Applied Sciences)
Show simple item record

Page view(s)

742
checked on Dec 22, 2024

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.