Please use this identifier to cite or link to this item: https://hdl.handle.net/10321/2962
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dc.contributor.authorNaidoo, Kameshneeen_US
dc.contributor.authorKumar, Ajiten_US
dc.contributor.authorSharma, Vikasen_US
dc.contributor.authorPermaul, Kugenen_US
dc.contributor.authorSingh, Surenen_US
dc.date.accessioned2018-05-15T07:02:15Z-
dc.date.available2018-05-15T07:02:15Z-
dc.date.issued2015-
dc.identifier.citationNaidoo, K. et al. 2015. Purification and characterization of an Endoinulinase from Xanthomonas campestris pv. phaseoli KM 24 Mutant. Food Technology and Biotechnology. 53(2): 146–153.en_US
dc.identifier.issn1330-9862-
dc.identifier.urihttp://hdl.handle.net/10321/2962-
dc.description.abstractAn extracellular endoinulinase from Xanthomonas campestris pv. phaseoli KM 24 mutant was purifi ed to homogeneity by gel fi ltration chromatography and showed a specifi c activ-ity of 119 U/mg. The optimum pH and temperature of the purifi ed enzyme were found to be 6.0 and 50 °C, respectively. The enzyme was stable up to 60 °C, retaining 60 % of residu-al activity for 30 min, but inactivated rapidly above 60 °C. The enzyme was found to be stable at pH=6–9 when it retained 100 % of its residual activity. The Lineweaver-Burk plot showed that the apparent Km and vmax values of the inulinase when using inulin as a sub-strate were 1.15 mg/mL and 0.15 μM/min, respectively, whereas the kcat value was found to be 0.145 min–1. The calculated catalytic effi ciency of the enzyme was found to be 0.126 (mg·min)/mL. The purifi ed inulinase can be used in the production of high fructose syr-ups.en_US
dc.format.extent8 pen_US
dc.language.isoenen_US
dc.publisherUniversity of Zagreben_US
dc.relation.ispartofFood technology and biotechnologyen_US
dc.subjectXanthomonas campestris pv. phaseoli KM 24 mutanten_US
dc.subjectInulinasesen_US
dc.subjectEndoinulinasesen_US
dc.subjectExoinulinasesen_US
dc.subjectFructooligosaccharidesen_US
dc.subjectInulinen_US
dc.titlePurification and characterization of an Endoinulinase from Xanthomonas campestris pv. phaseoli KM 24 Mutanten_US
dc.typeArticleen_US
dc.dut-rims.pubnumDUT-005734en_US
dc.identifier.doi10.17113/ft b.53.02.15.3902-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.fulltextWith Fulltext-
Appears in Collections:Research Publications (Applied Sciences)
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