Please use this identifier to cite or link to this item: https://hdl.handle.net/10321/336
DC FieldValueLanguage
dc.contributor.authorBenjamin, Sherilene Cherylen_US
dc.date.accessioned2008-09-04T06:54:47Z
dc.date.available2008-09-04T06:54:47Z
dc.date.issued2008-
dc.identifier.other314012-
dc.identifier.urihttp://hdl.handle.net/10321/336-
dc.descriptionThesis (Masters in Technology: Clinical Technology), Durban University of Technology, Durban, South Africa, 2008.en_US
dc.description.abstractAnaemia being one of the most severe complications of end stage renal disease is presently being managed with treatment by recombinant erythropoietin (RHuEPO). Recombinant erythropoietin (RHuEPO) produced by recombinant human DNA technology (RHuEPO) is becoming a standard part of therapy in chronic renal failure. In such patients anaemia is a leading cause of morbidity and mortality. Haemodialysis patients have grown dependent on RHuEPO therapy due their anaemia. The assessment of native erythropoietin levels and the detection of antibody levels in blood in the study were carried out to assist in the clear target of the treatment of anaemia. Haemoglobin levels were monitored over a trial period of six months during the RHuEPO therapy. Ferritin, transferrin saturation (T Sats %) and transferrin levels were monitored accordingly. Monitoring of erythropoietin levels in haemodialysis patients has been one of the first done in our population group in South Africa.Forty haemodialysis patients on RHuEPO therapy and ten haemodialysis patients not on RHuEPO therapy and ten healthy individuals from the Haemodialysis unit at Addington Hospital, Durban, South Africa were recruited to participate in the trial. Blood samples were collected then were centrifuged at 5 degrees celsius. Plasma was isolated, stored and subsequently used in enzyme linked immunosorbent assay (ELISA). Two ELISA were set up, one to measure EPO level and another for anti EPO antibodies. The dilutions of 1:50 were selected to detect the presence of antibodies. These have all been done in duplicates. Optical density of each sample was measured using a microplate reader at 450nm. The haemodialysis patients receiving RHuEPO presented with higher EPO levels as compared to the haemodialysis patients not receiving RHuEPO and the healthy individuals. However, in the study the HB levels were not increased over the trial period with higher RHuEPO doses. Higher doses of RHuEPO therapy showed no clear increase in haemoglobin levels. From a total of forty patients, twelve patients (35%) in the months of August and eight (25%) in the month of November were tested positive for antibodies to RHuEPO. Using statistical analysis, no correlation was observed between the antibody levels and the erythropoietin levels. However, we did not test whether the antibodies found were neutralizing or not. Bioassays for EPO may be used for that purpose.en_US
dc.format.extent178 pen_US
dc.language.isoenen_US
dc.subjectAnemia Treatmenten_US
dc.subject.lcshRecombinant erythropoietinen_US
dc.subject.lcshHemodialysis Patientsen_US
dc.subject.lcshErythropoietinen_US
dc.titleThe assessment of native erythropoietin and antibodies to recombinant erythropoietin in haemodialysis patientsen_US
dc.typeThesisen_US
dc.dut-rims.pubnumDUT-000285en_US
dc.description.levelMen_US
dc.identifier.doihttps://doi.org/10.51415/10321/336-
item.languageiso639-1en-
item.openairetypeThesis-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:Theses and dissertations (Health Sciences)
Files in This Item:
File Description SizeFormat
Benjamin_2008.pdf3.52 MBAdobe PDFThumbnail
View/Open
Show simple item record

Page view(s) 20

1,340
checked on Dec 22, 2024

Download(s) 20

1,292
checked on Dec 22, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.