Please use this identifier to cite or link to this item: https://hdl.handle.net/10321/3809
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dc.contributor.advisorIjabadeniyi, Oluwatosin Ademola-
dc.contributor.advisorPillai, Santhosh Kumar Kuttan-
dc.contributor.authorTakundwa, Brianmax Aubreyen_US
dc.date.accessioned2022-01-25T07:33:07Z-
dc.date.available2022-01-25T07:33:07Z-
dc.date.issued2020-
dc.identifier.urihttps://hdl.handle.net/10321/3809-
dc.descriptionSubmitted in fulfillment of the academic requirements for the Master of Applied Sciences in Food Science and Technology degree at the Department of Biotechnology and Food Science, Faculty of Applied Sciences Durban University of Technology, Durban, 2020.en_US
dc.description.abstractA marked increase in the prevalence of foodborne illnesses resulting in foodborne outbreaks globally have been noticed, primarily due to the contamination of foods with bacterial pathogens. Recently, there has been a growing interest in employing alternative decontamination methods against bacterial pathogens, as a result of problems associated with the commonly used chemical and thermal methods. Therefore, the current impetus is on employing decontamination techniques involving green technology (physical, biological) and hurdle technology. In this study, oregano, nisin and ultrasound treatments were employed on lettuce and cabbage at varying levels to elucidate their antimicrobial efficacy and subsequent effect on the physical and sensory properties of the vegetables. Specifically, fresh cut lettuce and cabbage samples were inoculated with Escherichia coli O157:H7 and Listeria monocytogenes and were subjected to a series of combined treatment applications, of nisin, oregano essential oil and ultrasound. The Box Behnken, response surface methodology (RSM) technique was used in formulating the various combination treatments that would, in turn, demonstrate the synergistic capabilities of the three factors, nisin, oregano and ultrasound when combined. Results from the RSM were then used in the optimization of the combined treatment parameters by maximizing the microbial log reduction in both ready-to-eat vegetables. The physical properties studied on lettuce and cabbage subjected to combined treatments were colour and texture, and their structural damage was investigated using electrolyte leakage. Sensory properties were also analyzed on non-inoculated cabbage samples previously subjected to combined antimicrobial treatments. The efficacy of nisin, oregano and ultrasound on the reduction of E. coli O157:H7 and L. monocytogenes on lettuce studied using RSM/Box-Behnken model design, was found to be reliable (p<0.05). The most effective treatment on both pathogens was a combination of 771.2 IU/g nisin, 0.185% v/v oregano and 14.65 min ultrasound which showed log reductions of 3.43 and 9.20 CFU/mL for E. coli O157:H7 and L. monocytogenes, respectively. Lettuce treatment with the combined antimicrobial treatments resulted in no significant differences in textural properties, specifically hardness. However, mild colour changes and a slight increase in the electrolyte leakage rate was observed, though they were within the permissible limits. The reduction of E. coli O157:H7 on cabbage was increased with the use of combined treatments of nisin, oregano and ultrasound. Hence, the combination (comprising 607.85 IU/g nisin, 0.20% v/v oregano and 14.98 min ultrasound) exhibited the highest log reduction of 3.66 CFU/mL. In the samples inoculated with L. monocytogenes, 731.25 IU/g nisin, 0.12% v/v oregano and 13.21 min ultrasound treatments were found to be the best combination exhibiting the highest log reduction of 8.27 CFU/mL. No significant colour and textural changes were observed between untreated and treated cabbage. However, a slight increase in the electrolyte leakage rate was observed after the application of the combined treatment. Sensory evaluation scores also had some factors that were slightly below par. Overall, results from the study demonstrated that a combination of nisin, oregano and ultrasound, is a promising alternative to chemical treatments for the reduction of E. coliO157:H7 and L. monocytogenes as well as retaining the quality characteristics of fresh produce. Prospectively, other studies could explore the frontier field of artificial intelligence and machine learning, in the form of predictive microbiology and mathematical modelling, in the fresh produce industry. This would present a better understanding of risk assessment that is powered by the technological advantage of data analytics.en_US
dc.format.extent158 pen_US
dc.language.isoenen_US
dc.subject.lcshAnti-infective agentsen_US
dc.subject.lcshEscherichia coli O157:H7--Controlen_US
dc.subject.lcshListeria monocytogenes--Controlen_US
dc.subject.lcshVegetables--Contamination--Preventionen_US
dc.subject.lcshFoodborne diseasesen_US
dc.titleAntimicrobial efficacy of nisin, oregano and ultrasound treatments against foodborne pathogens in ready-to-eat vegetablesen_US
dc.typeThesisen_US
dc.description.levelMen_US
dc.identifier.doihttps://doi.org/10.51415/10321/3809-
local.sdgSDG03-
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
item.openairetypeThesis-
item.grantfulltextopen-
item.cerifentitytypePublications-
Appears in Collections:Theses and dissertations (Applied Sciences)
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