Please use this identifier to cite or link to this item:
https://hdl.handle.net/10321/847
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Singh, Suren | en_US |
dc.date.accessioned | 2013-04-08T07:17:25Z | - |
dc.date.available | 2013-04-08T07:17:25Z | - |
dc.date.issued | 2012-06-08 | - |
dc.identifier.citation | Li Zhou, Dan-Dan Niu, Kang-Ming Tian, Xian-Zhong Chen, Bernard A. Prior, Wei Shen, Gui-Yang Shi, Suren Singh, Zheng-Xiang Wang. 'Genetically switched d-lactate production in Escherichia coli.' Metabolic Engineering. 14,5 (2012): 560-568. 10.1016/j.ymben.2012.05.004. | en_US |
dc.identifier.uri | http://hdl.handle.net/10321/847 | - |
dc.description.abstract | During a fermentation process, the formation of the desired product during the cell growth phase competes with the biomass for substrates or inhibits cell growth directly, which results in a decrease in production efficiency. A genetic switch is required to precisely separate growth from production and to simplify the fermentation process. The ldhA promoter, which encodes the fermentative d-lactate dehydrogenase (LDH) in the lactate producer Escherichia coli CICIM B0013-070 (ack-pta pps pflB dld poxB adhE frdA), was replaced with the λ pR and pL promoters (as a genetic switch) using genomic recombination and the thermo-controllable strain B0013–070B (B0013-070, ldhAp::kan-cIts857-pR–pL), which could produce two-fold higher LDH activity at 42 °C than the B0013-070 strain, was created. When the genetic switch was turned off at 33 °C, strain B0013-070B produced 10% more biomass aerobically than strain B0013-070 and produced only trace levels of lactate which could reduce the growth inhibition caused by oxygen insufficiency in large scale fermentation. However, 42 °C is the most efficient temperature for switching on lactate production. The volumetric productivity of B0013-070B improved by 9% compared to that of strain B0013-070 when it was grown aerobically at 33 °C with a short thermo-induction at 42 °C and then switched to the production phase at 42 °C. In a bioreactor experiment using scaled-up conditions that were optimized in a shake flask experiment, strain B0013-070B produced 122.8 g/l d-lactate with an increased oxygen-limited productivity of 0.89 g/g·h. The results revealed the effectiveness of using a genetic switch to regulate cell growth and the production of a metabolic compound. | en_US |
dc.format.extent | 9 | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.subject | Genetic switch | en_US |
dc.subject | Thermo-regulation | en_US |
dc.subject | d-Lactate production | en_US |
dc.subject | Lambda pL and pR tandem promoters | en_US |
dc.subject | LdhA | en_US |
dc.subject.lcsh | Escherichia coli | en_US |
dc.subject.lcsh | Lactate dehydrogenase | en_US |
dc.title | Genetically switched D-lactate production in Escherichia coli | en_US |
dc.type | Article | en_US |
dc.publisher.uri | http://www.sciencedirect.com/science/article/pii/S1096717612000651 | en_US |
dc.dut-rims.pubnum | DUT-001786 | en_US |
dc.identifier.doi | https://doi.org/10.1016/j.ymben.2012.05.004 | - |
item.openairetype | Article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.fulltext | No Fulltext | - |
Appears in Collections: | Research Publications (Applied Sciences) |
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