Biodiesel production potential of wastewater microalgae chlorella SP. under photoautotrophic and heterotrophic growth conditions

Abstract

In the present study, a microalgae (Chlorella sp.) isolated from wastewater pond has been studied in both photoautotrophic and heterotrophic growth conditions in bioreactor to evaluate the cell growth rate and lipid content for biodiesel production. Maximum amount of biomass was recovered from the bioreactor of Chlorella sp. grown under heterotrophic growth conditions with 8.90 gL-1 compared to photoautotrophic growth conditions, which was almost 3.6, fold lesser than the former. Heterotrophic growth of Chlorella sp. resulted in the accumulation of high lipid content in cells compared autotrophic growth by enhancing lipid production by 4.4 fold. The results suggested that heterotrophic growth of microalgae is an efficient method for the production of biomass and high lipid content in the cells, which can reduce the cost of microalgal biomass production and microalgal oil production. The quality of the oil produced from the cells of heterotrophic growth is also superior compared the oil from photoautotrophic growth.