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Functional characterisation of heterotrophic denitrifying bacteria in wastewater treatment systems

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dc.contributor.author Ramdhani, Nishani
dc.date.accessioned 2008-07-16T12:46:59Z
dc.date.available 2008-07-16T12:46:59Z
dc.date.issued 2005
dc.identifier.other DIT112619
dc.identifier.uri http://hdl.handle.net/10321/311
dc.description Thesis (M.Tech.: Biotechnology)-Dept. of Biotechnogy, Durban Institute of Technology, 2005 xvi, 85 leaves : ill. ; 31 cm en_US
dc.description.abstract Atmospheric nitrogen pollution is on the increase and human activities are directly or indirectly responsible for the generation of the various nitrogen polluting compounds. This can lead to the two major problems of eutrophication and groundwater pollution. Therefore, the removal of nutrients such as nitrogen and phosphorus from wastewater is important. Nitrogen removal from wastewater is achieved by a combination of nitrification and denitrification. Thus, there is a need to identify and characterise heterotrophic denitrifying bacteria involved in denitrification in wastewater treatment systems. The aim of this study, therefore, was to characterise heterotrophic denitrifying bacteria through detailed biochemical and molecular analysis, to facilitate the understanding of their functional role in wastewater treatment systems. Drysdale (2001) isolated heterotrophic denitrifiers to obtain a culture collection of 179 isolates. This culture collection was used to screen for nitrate and nitrite reduction using the colorimetric biochemical nitrate reduction test. The isolates were thereafter Gram stained to assess their gram reaction, cellular and colonial morphology. Based on these results identical isolates were discarded and a culture collection of approximately 129 isolates remained. The genetic diversity of the culture collection was investigated by the analysis of polymerase chain reaction (PCR)-amplified 16S ribosomal DNA (rDNA) fragments on polyacrylamide gels using denaturing gradient gel electrophoresis (DGGE). Thus DNA fragments of the same length but different nucleotide sequences were effectively separated and microbial community profiles of eight predominant isolates were created. Batch experiments were conducted on these eight isolates, the results of which ultimately confirmed their characterisation and placed them into their four functional groups i.e. 3 isolates were incomplete denitrifiers, 2 isolates were true denitrifiers, 2 isolates were sequential denitrifiers and 1 isolate was an exclusive nitrite reducer. en_US
dc.language.iso en en_US
dc.subject Biotechnology--Dissertations, Academic en_US
dc.subject Sewage--Purification en_US
dc.subject Sanitary microbiology en_US
dc.subject Sewage--Microbiology en_US
dc.subject Bacteria, denitrifying en_US
dc.subject Denitrification en_US
dc.title Functional characterisation of heterotrophic denitrifying bacteria in wastewater treatment systems en_US
dc.type Thesis en_US
dc.dut-rims.pubnum DUT-000023


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