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|Title:||Biocatalytic conversion of lipids from microalgae Scenedesmus obliquus to biodiesel using Pseudomonas ﬂuorescens lipase||Authors:||Guldhe, Abhishek
|Keywords:||Biodiesel;Microalgae;Lipase;Scenedesmus obliquus;Pseudomonas fluorescens||Issue Date:||31-Jan-2015||Publisher:||Elsevier||Source:||Guldhe, A. et al. 2015. Biocatalytic conversion of lipids from microalgae Scenedesmus obliquus to biodiesel using Pseudomonas ﬂuorescens lipase. Fuel. 147(1): 117-124.||Abstract:||Conversion of microalgal lipids using biocatalyst is a novel and greener approach to produce biodiesel. Free and immobilized lipases from Candida sp. and Pseudomonas ﬂuorescens along with free lipases from porcine pancreas and wheat germ were screened for biodiesel conversion of Scenedesmus obliquus lipids. Among selected lipases from various sources immobilized lipase from P. ﬂuorescens showed superior biodiesel conversion. Optimization of reaction parameters viz. lipase amount, temperature, methanol to oil molar ratio and water content was carried out using response surface methodology. Best conversion of 66.55% was achieved at 35 C, methanol to oil ratio of 3:1 with 10% enzyme amount and 2.5% water content based on oil weight. To tackle methanol tolerance step-wise methanol addition was applied, which improved biodiesel conversion upto 90.81%. Immobilized P. ﬂuorescens lipase can be used for 4 batches without much loss in conversion efﬁciency (>95%). Biodiesel produced has the cetane number of 51.77, Caloriﬁc value of 37.67 MJ kg1. Most of the fuel properties of biodiesel met the speciﬁcations set by ASTM and EN standards.||URI:||http://hdl.handle.net/10321/1650||ISSN:||0016-2361|
|Appears in Collections:||Research Publications (Applied Sciences)|
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