Please use this identifier to cite or link to this item: http://hdl.handle.net/10321/1650
Title: Biocatalytic conversion of lipids from microalgae Scenedesmus obliquus to biodiesel using Pseudomonas fluorescens lipase
Authors: Guldhe, Abhishek 
Singh, Bhaskar 
Rawat, Ismail 
Perumal, Kugen 
Bux, Faizal 
Keywords: Biodiesel;Microalgae;Lipase;Scenedesmus obliquus;Pseudomonas fluorescens
Issue Date: 31-Jan-2015
Publisher: Elsevier
Source: Guldhe, A. et al. 2015. Biocatalytic conversion of lipids from microalgae Scenedesmus obliquus to biodiesel using Pseudomonas fluorescens lipase. Fuel. 147(1): 117-124.
Abstract: Conversion of microalgal lipids using biocatalyst is a novel and greener approach to produce biodiesel. Free and immobilized lipases from Candida sp. and Pseudomonas fluorescens along with free lipases from porcine pancreas and wheat germ were screened for biodiesel conversion of Scenedesmus obliquus lipids. Among selected lipases from various sources immobilized lipase from P. fluorescens showed superior biodiesel conversion. Optimization of reaction parameters viz. lipase amount, temperature, methanol to oil molar ratio and water content was carried out using response surface methodology. Best conversion of 66.55% was achieved at 35 C, methanol to oil ratio of 3:1 with 10% enzyme amount and 2.5% water content based on oil weight. To tackle methanol tolerance step-wise methanol addition was applied, which improved biodiesel conversion upto 90.81%. Immobilized P. fluorescens lipase can be used for 4 batches without much loss in conversion efficiency (>95%). Biodiesel produced has the cetane number of 51.77, Calorific value of 37.67 MJ kg􀀁1. Most of the fuel properties of biodiesel met the specifications set by ASTM and EN standards.
URI: http://hdl.handle.net/10321/1650
ISSN: 0016-2361
Appears in Collections:Research Publications (Applied Sciences)

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