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Title: Physico-chemical and biotic factors influencing microalgal seed culture propagation for inoculation of a large scale raceway pond
Authors: Mutanda, Taurai 
Ranjith Kumar, R. 
Bux, Faizal 
Keywords: Biodiesel;Biomass;Inoculum;Lipid;Raceway pond;Seed culture
Issue Date: 2014
Publisher: African Journals Online
Source: Mutanda, T, Ranjith, R.K. and Bux, F. 2014. Physico-chemical and biotic factors influencing microalgal seed culture propagation for inoculation of a large scale raceway pond. African Journal of Biotechnology. 13(31): 3607-3616.
Journal: African journal of biotechnology 
Abstract: The growth of Chlorella vulgaris in open pond aquatic conditions poses serious challenges due to the interplay of both physico-chemical and biotic factors. We report here the monitoring of physico-chemical and biotic parameters affecting the propagation of C. vulgaris seed culture for inoculation of a large scale raceway pond (300 000 L capacity) in South Africa. The C. vulgarisstrain used for this purpose was isolated from a wastewater maturation pond and characterized for its potential for biomass and lipid production. The isolate was grown aseptically in 4 × 25 L aspirator bottles in BG-11 medium under ambient laboratory conditions and the culture was supplied with filtered air and exposed to 200 µmol photons per m2 per second using Gro-Lux agricultural fluorescent lights. The culture was transferred to a 500 L capacity portable pool under open conditions. This pond was used to further inoculate 3 more portable ponds. Physico-chemical and biotic growth parameters were monitored on a daily basis in the three ponds. The over reliance on fossil fuels will have a major impact on power supply in the near future if renewable sources of energy are not developed at a fast pace. The developed inoculum was subsequently used to inoculate an open raceway pond for large scale biomass production for biodiesel production.
ISSN: 1684-5315
Appears in Collections:Research Publications (Applied Sciences)

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