Please use this identifier to cite or link to this item:
https://hdl.handle.net/10321/307
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Permaul, Kugen | - |
dc.contributor.author | Pienaar, Fernando | en_US |
dc.date.accessioned | 2008-07-16T12:46:25Z | - |
dc.date.available | 2008-07-16T12:46:25Z | - |
dc.date.issued | 2007 | - |
dc.identifier.other | 308421 | - |
dc.identifier.uri | http://hdl.handle.net/10321/307 | - |
dc.description | Submitted in fulfilment of the requirements for the Degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2007. | en_US |
dc.description.abstract | The development of transgenic plants resulted in the need to utilize the various molecular methods (e.g., ELISA, real - time PCR etc.) for the detection or analysis of the presence or absence of a specific trait in a particular plant (Bt in this study). The overall aim of this study was to optimize a half – seed extraction technique as part of a laboratory protocol for transgenic maize plants and to explore the possibility of using the following molecular techniques: horizontal isoelectric focusing, real - time PCR and ELISA, as methods for detection of the Bt trait for incorporation into the half – seed extraction protocol. | en_US |
dc.format.extent | 81 p | en_US |
dc.language.iso | en | en_US |
dc.subject | Corn | en_US |
dc.subject | Crops | en_US |
dc.subject | Plant genetic engineering | en_US |
dc.subject | Crop improvement | en_US |
dc.subject | Biotechnology--Dissertations, Academic | en_US |
dc.subject.lcsh | Corn--Genetic engineering | en_US |
dc.subject.lcsh | Crops--Genetic engineering | en_US |
dc.subject.lcsh | Corn--Biotechnology | en_US |
dc.title | Comparative analysis of genetically modified maize by implementation of a half-seed extraction technique | en_US |
dc.type | Thesis | en_US |
dc.description.level | M | en_US |
dc.identifier.doi | https://doi.org/10.51415/10321/307 | - |
item.languageiso639-1 | en | - |
item.openairetype | Thesis | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
Appears in Collections: | Theses and dissertations (Applied Sciences) |
Files in This Item:
File | Description | Size | Format | |
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Pienaar_2007.pdf | 789.95 kB | Adobe PDF | View/Open |
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