Please use this identifier to cite or link to this item: https://hdl.handle.net/10321/3183
DC FieldValueLanguage
dc.contributor.advisorPermaul, Kugen-
dc.contributor.advisorSingh, Suren-
dc.contributor.authorZininga, Johnson Tungamiraien_US
dc.date.accessioned2018-10-29T08:21:27Z-
dc.date.available2018-10-29T08:21:27Z-
dc.date.issued2018-
dc.identifier.other700940-
dc.identifier.urihttp://hdl.handle.net/10321/3183-
dc.descriptionSubmitted in complete fulfillment for the Degree of Master of Applied Science in Biotechnology, Durban University of Technology, Durban, South Africa, 2018.en_US
dc.description.abstractFilamentous fungi are well-known sources of a wide variety of industrially-useful biomolecules. This study demonstrates the applicability of a newly isolated oleaginous fungi Mucor circinelloides ZSKP for lipid and chitosan production. Parameters affecting co-production were identified and were statistically optimized, which resulted in a 3–fold improvement in lipid production. The lipid profile showed a high content of unsaturated fatty acids including oleic, linolenic and linoleic acids, while palmitic acid was the major saturated fatty acid (21%). A comparative study to evaluate the efficacy of enzymatic and chemical treatments for biodiesel production from fungal lipids and sunflower oil revealed slightly enhanced production of biodiesel from fungal lipids, using a commercial lipase. The biodiesel synthesized using lipids from M. circinelloides ZSKP satisfied standard specifications and had a higher cetane number (56), lower kinematic viscosity (4.6 mm2/s) and lower acid number (0.03) compared to sunflower oil. Upon optimizing chitosan production and extraction processes the chitosan production was improved 2-fold. The fungal chitosan showed antimicrobial properties and was more effective against Aspergillus niger A chitosan spray was developed which was able to increase the shelf life of fresh fruit produce. These results indicate that Mucor circinelloides ZSKP is a promising candidate for concurrent production of lipids and the versatile bio-polymer chitosan.en_US
dc.format.extent104 pen_US
dc.language.isoenen_US
dc.subject.lcshChitosan--Biotechnologyen_US
dc.subject.lcshChitinen_US
dc.subject.lcshLipidsen_US
dc.subject.lcshMucoraceaeen_US
dc.subject.lcshMucoren_US
dc.titleProduction of chitosan and lipids from a newly isolated Mucor circinelloidesen_US
dc.typeThesisen_US
dc.description.levelMen_US
dc.identifier.doihttps://doi.org/10.51415/10321/3183-
local.sdgSDG07-
item.languageiso639-1en-
item.openairetypeThesis-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:Theses and dissertations (Applied Sciences)
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