Please use this identifier to cite or link to this item: https://hdl.handle.net/10321/4738
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dc.contributor.authorMtetwa, Hlengiwe N.en_US
dc.contributor.authorAmoah, Isaac Dennisen_US
dc.contributor.authorKumari, Sheenaen_US
dc.contributor.authorBux, Faizalen_US
dc.contributor.authorReddy, Poovendhreeen_US
dc.date.accessioned2023-04-26T09:25:27Z-
dc.date.available2023-04-26T09:25:27Z-
dc.date.issued2021-11-
dc.identifier.citationMtetwa, H.N. et al. 2021. Wastewater-based surveillance of antibiotic resistance genes associated with tuberculosis treatment regimen in KwaZulu Natal, South Africa. Antibiotics-Basel. 10(11): 1362-. doi:10.3390/antibiotics10111362en_US
dc.identifier.issn2079-6382-
dc.identifier.issn2079-6382 (Online)-
dc.identifier.otherisidoc: XF9SU-
dc.identifier.otherpubmed: 34827300-
dc.identifier.urihttps://hdl.handle.net/10321/4738-
dc.description.abstractEssential components of public health include strengthening the surveillance of infectious diseases and developing early detection and prevention policies. This is particularly important for drug-resistant tuberculosis (DR-TB), which can be explored by using wastewater-based surveillance. This study aimed to use molecular techniques to determine the occurrence and concentration of antibiotic-resistance genes (ARGs) associated with tuberculosis (TB) resistance in untreated and treated wastewater. Raw/untreated and treated (post-chlorination) wastewater samples were taken from three wastewater treatment plants (WWTPs) in South Africa. The ARGs were selected to target drugs used for first- and second-line TB treatment. Both conventional polymerase chain reaction (PCR) and the more advanced droplet digital PCR (ddPCR) were evaluated as surveillance strategies to determine the distribution and concentration of the selected ARGs. The most abundant ARG in the untreated wastewater was the rrs gene, associated with resistance to the aminoglycosides, specifically streptomycin, with median concentration ranges of 4.69-5.19 log copies/mL. In contrast, pncA gene, associated with resistance to the TB drug pyrazinamide, was the least detected (1.59 to 2.27 log copies/mL). Resistance genes associated with bedaquiline was detected, which is a significant finding because this is a new drug introduced in South Africa for the treatment of multi-drug resistant TB. This study, therefore, establishes the potential of molecular surveillance of wastewater for monitoring antibiotic resistance to TB treatment in communities.en_US
dc.format.extent16 pen_US
dc.format.mediumElectronic-
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofAntibiotics-Basel; Vol. 10, Issue 11en_US
dc.subjectMultidrug-resistant tuberculosisen_US
dc.subjectAntibiotic resistant genesen_US
dc.subjectWastewateren_US
dc.subjectDroplet digital PCRen_US
dc.subjectWastewater-based epidemiologyen_US
dc.subjectAntibiotic resistant genesen_US
dc.subjectDroplet digital PCRen_US
dc.subjectMultidrug-resistant tuberculosisen_US
dc.subjectWastewateren_US
dc.subjectWastewater-based epidemiologyen_US
dc.titleWastewater-based surveillance of antibiotic resistance genes associated with tuberculosis treatment regimen in KwaZulu Natal, South Africaen_US
dc.typeArticleen_US
dc.date.updated2023-04-13T08:52:13Z-
dcterms.dateAccepted2021-10-28-
dc.identifier.doi10.3390/antibiotics10111362-
local.sdgSDG06-
local.sdgSDG03-
local.sdgSDG15-
item.languageiso639-1en-
item.openairetypeArticle-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:Research Publications (Health Sciences)
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