Please use this identifier to cite or link to this item: https://hdl.handle.net/10321/4739
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dc.contributor.authorMtetwa, Hlengiwe N.en_US
dc.contributor.authorAmoah, Isaac Dennisen_US
dc.contributor.authorKumari, Sheenaen_US
dc.contributor.authorBux, Faizalen_US
dc.contributor.authorReddy, Poovendhreeen_US
dc.date.accessioned2023-04-26T09:31:32Z-
dc.date.available2023-04-26T09:31:32Z-
dc.date.issued2022-02-
dc.identifier.citationMtetwa, H.N. et al. 2022. Molecular surveillance of tuberculosis-causing mycobacteria in wastewater. Heliyon 8(2): e08910-. doi:10.1016/j.heliyon.2022.e08910en_US
dc.identifier.issn2405-8440-
dc.identifier.issn2405-8440 (Online)-
dc.identifier.otherisidoc: ZQ6PR-
dc.identifier.otherpubmed: 35198775-
dc.identifier.urihttps://hdl.handle.net/10321/4739-
dc.description.abstractThe surveillance of tuberculosis infections has largely depended on clinical diagnostics and hospitalization data. The advancement in molecular methods creates an opportunity for the adoption of alternative surveillance systems, such as wastewater-based epidemiology. This study presents the use of conventional and advanced polymerase chain reaction techniques (droplet digital PCR) to determine the occurrence and concentration of total mycobacteria and members of the <i>Mycobacterium tuberculosis</i> complex (MTBC) in treated and untreated wastewater. Wastewater samples were taken from three wastewater treatment plants (WWTPs) in the city of Durban, South Africa, known for a high burden of TB/MDR-TB due to HIV infections. All untreated wastewater samples contained total mycobacteria and MTBC at varying percentages per WWTP studied. Other members of the MTBC related to tuberculosis infection in animals, <i>M. bovis</i> and <i>M. caprae</i> were also detected. The highest median concentration detected in untreated wastewater was up to 4.9 (±0.2) Log10 copies/ml for total mycobacteria, 4.0 (±0.85) Log10 copies/ml for MTBC, 3.9 (±0.54) Log10 copies/ml for <i>M. tuberculosis</i>, 2.7 (±0.42) Log10 copies/ml for <i>M. africanum</i>, 4.0 (±0.29) Log10 copies/ml for <i>M. bovis</i> and 4.5 (±0.52) Log10 copies/ml for <i>M. caprae</i>. Lower concentrations were detected in the treated wastewater, with a statistically significant difference (P-value ≤ 0.05) in concentrations observed. The log reduction achieved for these bacteria in the respective WWTPs was not statistically different, indicating that the treatment configuration did not have an impact on their removal. The detection of <i>M. africanum</i> in wastewater from South Africa shows that it is possible that some of the TB infections in the community could be caused by this mycobacterium. This study, therefore, highlights the potential of wastewater-based epidemiology for monitoring tuberculosis infections.en_US
dc.format.extent9 pen_US
dc.format.mediumElectronic-eCollection-
dc.language.isoenen_US
dc.publisherElsevier BVen_US
dc.relation.ispartofHeliyon; Vol. 8, Issue 2en_US
dc.subjectTuberculosisen_US
dc.subjectMycobacteriaen_US
dc.subjectMycobacterium tuberculosis complexen_US
dc.subjectWastewateren_US
dc.subjectDroplet digital PCRen_US
dc.subjectMycobacteriaen_US
dc.subjectMycobacterium tuberculosis complexen_US
dc.subjectTuberculosisen_US
dc.subjectWastewateren_US
dc.titleMolecular surveillance of tuberculosis-causing mycobacteria in wastewateren_US
dc.typeArticleen_US
dc.date.updated2023-04-13T08:52:29Z-
dcterms.dateAccepted2022-2-2-
dc.identifier.doi10.1016/j.heliyon.2022.e08910-
local.sdgSDG06-
local.sdgSDG15-
local.sdgSDG11-
local.sdgSDG03-
item.languageiso639-1en-
item.openairetypeArticle-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:Research Publications (Health Sciences)
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