Please use this identifier to cite or link to this item: https://hdl.handle.net/10321/5160
Title: Bioaugmentation efficiency of diesel degradation by Bacillus pumilus JLB and Acinetobacter calcoaceticus LT1 in contaminated soils
Authors: Pillay, Charlene
Lin, Johnson 
Keywords: Diesel;06 Biological Sciences;10 Technology;Biotechnology;Bioremediation;Bioattenuation;Bioaugmentation;Biostimulation
Issue Date: 11-Oct-2010
Source: Pillay, C. and Lin, J. 2010. Bioaugmentation efficiency of diesel degradation by Bacillus pumilus JLB and Acinetobacter calcoaceticus LT1 in contaminated soils. African Journal of Biotechnology. 9(41): 6881-6888. doi:10.5897/AJB09.829
Journal: African Journal of Biotechnology; Vol. 9, Issue 41 
Abstract: 
The abilities of diesel-degrading Bacillus pumilus JLB and Acinetobacter calcoaceticus LT1 were tested
in contaminated soils. The effect of nutrient supplementation on bioaugmented samples was also
examined. The results show that bio-augmentation and biostimulation accelerated significantly (p <
0.05) the diesel degradation in the contaminated loam soil and sea sand. Supplementing fertilizers to the
augmented loam samples did not result in a significantly higher degradation rate. Furthermore, A.
calcoaceticus LT1 alone failed to stimulate higher degradation rates in sea sand unless further
supplementation of fertilizer. The results from environmental scanning electron microscopy
demonstrate the population increases, then decreases in augmented samples corresponding to the
level of diesel degradation. Fungi-like microorganisms became dominant in contaminated loam soil at
the end of the study but not in sea sand. The study shows that it is critical not only to understand the
physiology of the inoculum but also how it affects microbial community structure and function before
the microorganism being introduced in the contaminated soil.
URI: https://hdl.handle.net/10321/5160
ISSN: 1684-5315
DOI: 10.5897/AJB09.829
Appears in Collections:Research Publications (Applied Sciences)

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