Please use this identifier to cite or link to this item: https://hdl.handle.net/10321/5442
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dc.contributor.advisorMellem, John Jason-
dc.contributor.authorMohan, Naazneeen_US
dc.date.accessioned2024-08-31T15:53:05Z-
dc.date.available2024-08-31T15:53:05Z-
dc.date.issued2024-05-
dc.identifier.urihttps://hdl.handle.net/10321/5442-
dc.descriptionSubmitted in fulfilment of the requirements of the degree of Master of Applied Sciences in Biotechnology and Food Sciences, Durban University of Technology, Durban, South Africa, 2024.en_US
dc.description.abstractSilver nanoparticles have been proven to have anticancer abilities but they have been known to agglomerate and become toxic. Therefore, various studies have been conducted to explore ways of preventing aggregation using biopolymers such as starch. This study makes use of Lablab purpureus (hyacinth bean) porous starch to biosynthesize and encapsulate silver nanoparticles and then test its anticancer potential. Porous starches were produced from hyacinth bean using three different techniques. These were compared against the native starch with silver nanoparticles, then synthesized and encapsulated using the porous starch. In comparison to the native starch, the porous starches made through solvent exchange and enzyme hydrolysis had similar outcomes with granules exhibiting pores, as shown by the structural and chemical characteristics. The lack of pasting properties and extremely distinct chemical and structural graphs of the porous starch, produced by freeze-thaw procedures, may be related to the presence of mercaptosuccinic acid. It was decided to employ porous starch made by solvent-exchange (SE) for the manufacture of silver nanoparticles as it contained resistant starch. Nanoparticles were produced using the porous starch from solvent-exchange, characterised and tested for their anticancer potential. Silver nanoparticles were indicative of a colour change from clear to brown, as well as, the characteristic peak at 425 nm for silver nanoparticle formation. Silver nanoparticles were implanted into porous starch at a size of around 50 nm, as further evidenced by the particle size distribution and TEM images of spherical granules with dark spots within. The zeta potential for the silver nanoparticles was -34 mV, thereby indicating that aggregation was minimized and particles were stable. The nanoparticles demonstrated less cytotoxicity in the human colon (CACO) and cervical (HELA) cancer cell lines, but more inhibition in the human breast (MCF-7) cancer cell line than the positive control camptothecin. The human muscle (C2C12), normal cell line's capacity to sustain cell viability for silver nanoparticles demonstrated that AgNP were not toxic. However, to maximize the potential of the silver nanoparticles implanted in porous starch, more research is necessary.en_US
dc.format.extent90 pen_US
dc.language.isoenen_US
dc.subjectSilver nanoparticlesen_US
dc.subjectAnticancer abilitiesen_US
dc.subject.lcshCancer--Treatmenten_US
dc.subject.lcshAntineoplastic agentsen_US
dc.subject.lcshAsparagaceaeen_US
dc.subject.lcshPlant biotechnologyen_US
dc.titleAnticancer activity of silver nanoparticles embedded in porous starch as a potential delivery systemen_US
dc.typeThesisen_US
dc.description.levelMen_US
dc.identifier.doihttps://doi.org/10.51415/10321/5442-
local.sdgSDG11en_US
local.sdgSDG16en_US
item.fulltextWith Fulltext-
item.openairetypeThesis-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:Theses and dissertations (Applied Sciences)
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